Nitrate assimilates into the root where the conversion of nitrate to nitrite in the cytosol, a reduction reaction that involves the transfer of two electrons catalyzes by an enzyme that is NAD(P)H dependent is known as

TLS Online TPP Program

#Question id: 38731

#Plant Biotechnology

Q.Regarding epidermis removal during leaf preparation:

1. It improves penetration of cell wall–degrading enzymes.

2. It removes cells with lower regenerative capacity.

3. It prevents plasmolysis during enzyme treatment.

4. It exposes metabolically active mesophyll cells.

Which of the above statements are correct?

TLS Online TPP Program

#Question id: 38732

#Plant Biotechnology

Q. With reference to plasmolysis prior to enzymatic digestion:

1. It facilitates separation of plasma membrane from cell wall.

2. It reduces mechanical damage during wall digestion.

3. It is induced by placing tissues in hypotonic solution.

4.  It enhances enzyme accessibility to cellulose microfibrils.

Which statements are correct?

TLS Online TPP Program

#Question id: 38733

#Plant Biotechnology

Q. Concerning enzymatic isolation of protoplasts:

1. Cellulase hydrolyzes β-1,4-glycosidic bonds of cellulose.

2. Pectinase degrades the middle lamella.

3. Excessive enzyme exposure leads to osmotic bursting.

4. Enzyme treatment preserves plasmodesmata.

Which statements are correct?

TLS Online TPP Program

#Question id: 38734

#Plant Biotechnology

Q.  Regarding density gradient centrifugation used in protoplast purification:

1. Separation is based on buoyant density.

2. Viable protoplasts form a distinct band.

3. Broken cells sediment faster than intact protoplasts.

4. The method separates cells based on size alone.

Which statements are correct?

TLS Online TPP Program

#Question id: 38735

#Plant Biotechnology

Q. With reference to feeder layer technique in protoplast culture:

1. Feeder cells provide diffusible growth factors.

2. It compensates for the absence of cell-to-cell signaling.

3. Feeder cells directly fuse with protoplasts.

4. It increases plating efficiency of isolated protoplasts.

Which statements are correct?

TLS Online TPP Program

#Question id: 38736

#Plant Biotechnology

Q. Regarding callus formation from protoplasts:

1. Callus formation requires prior regeneration of the cell wall.

2. Callus cells are genetically identical to the donor plant.

3. Callus represents a dedifferentiated state.

4. High auxin concentration promotes callus induction.

Which statements are correct?