Ammonium can be assimilated via an alternative pathway rather than primary pathway which involves the sequential actions of glutamine synthetase(GS) and glutamate synthase(GOGAT), these alternative pathway catalyzes by enzyme known as,

TLS Online TPP Program

#Question id: 39280

#Plant Biotechnology

Q. Regarding cybridization for male sterile line development:

Statement I: Cybrids allow rapid transfer of mitochondria even across sexually incompatible species.

Statement II: Male sterile lines developed via protoplast fusion can be used for hybrid seed production using CMS restorers.

Statement III: Nuclear genomes are exchanged between donor and recipient protoplasts in cybridization.

Which statements are correct?

TLS Online TPP Program

#Question id: 39281

#Plant Biotechnology

Q. Statement I: Conventional backcrossing is a more time-efficient method for cytoplasm transfer compared to somatic hybridization. 

Statement II: Protoplast fusion can achieve cytoplasm transfer in approximately one year.

TLS Online TPP Program

#Question id: 39282

#Plant Biotechnology

Q. Statement I: In the cybrid approach, iodoacetate is used to inactivate the nuclei of the recipient strain (Parent A). 

Statement II: Irradiation is used to inactivate the nuclei of the donor strain (Parent B) to ensure only its cytoplasm is transferred.

TLS Online TPP Program

#Question id: 39283

#Plant Biotechnology

Q. Statement I: In the fusion product described, the mitochondria are always derived from the donor strain (Parent B).

 Statement II: The chloroplasts in the resulting regenerants must always come from the recipient strain (Parent A)

TLS Online TPP Program

#Question id: 39284

#Plant Biotechnology

Q. Statement I: Fusing tomato protoplasts with irradiated Solanum acaule protoplasts produced plants that were morphologically identical to Solanum acaule. 

Statement II: The resulting tomato regenerants exhibited Cytoplasmic Male Sterility (CMS), which was inherited maternally.

TLS Online TPP Program

#Question id: 39285

#Plant Biotechnology

Q. Statement I: Protoplast fusion can be used to develop male sterile lines even between sexually incompatible species. 

Statement II: CMS lines developed through this method cannot be used with traditional CMS restorers.