One of the enzyme that are involve in the ammonium assimilation is Asparagine synthetase (AS), some statements given below about AS such as,

a) This enzyme that catalyzes this reaction, is found in the cytosol of leaves and roots and in nitrogen-fixing nodules

b) High levels of light and carbohydrate inhibit the expression of genes coding for AS and the activity of the enzyme

c) Conditions of ample energy stimulate AS, thus they favor nitrogen assimilation into glutamine and glutamate

d) Energy-limited conditions inhibit GS and GOGAT, stimulate AS, and thus favor nitrogen assimilation into asparagine, a compound that is rich in nitrogen and sufficiently stable for long-distance transport or long term storage

Which of the following statements about AS is correct?

#Question id: 40414

#Applied Microbiology

1. Read the following statements regarding the Gram staining technique.
1. 1. Statement I: Heat fixing is done after adding the crystal violet stain to the slide.
2. 1. Statement II: Ideally, the iodine solution (mordant) should be kept on the smear for approximately 1 minute.

#Question id: 40415

#Applied Microbiology

Which of the following errors during the Gram staining procedure would most likely cause a Gram-positive bacterium to appear Gram-negative (pink)?

#Question id: 40416

#Applied Microbiology

In a eukaryotic cell utilizing the glycerol-3-phosphate shuttle, what is the total yield of ATP via oxidative phosphorylation alone from the complete oxidation of 1 molecule of glucose? (Assume 1 NADH = 2.5 ATP and 1 FADH₂ = 1.5 ATP)

#Question id: 40417

#Applied Microbiology

Consider the following statements regarding ATP production during aerobic respiration:

1. Substrate-level phosphorylation occurs in both the cytoplasm and the mitochondrial matrix.

2. The oxidation of one molecule of Acetyl-CoA in the Citric Acid Cycle results in 11 ATPs via oxidative phosphorylation.

3. The enzyme ATP Synthase (Complex V) utilizes the proton motive force to generate ATP in the intermembrane space.

#Question id: 40418

#Applied Microbiology

Assertion (A) : The net gain of ATP during glycolysis is only 2 ATP, even though 4 ATP molecules are actually produced.

Reason (R): Two molecules of ATP are consumed during the preparatory phase of glycolysis to phosphorylate glucose and fructose-6-phosphate.

#Question id: 40419

#Applied Microbiology

 Match the metabolic stage (Column I) with the correct ATP/Reduced Coenzyme yield per glucose molecule (Column II)