Metaphase chromosomes can be identified

a. by shape.                 

b. by the size and number of introns.

c. by banding patterns with Giemsa reagent.

d. by chromosome painting.

TLS Online TPP Program

#Question id: 39288

#Plant Biotechnology

Q. Assertion: Cybrids allow for the combination of mitochondria from one species with chloroplasts from a different species.

 Reason: This combination can be used to overcome deleterious effects, such as chlorophyll deficiency, found in some alloplasmic lines.

TLS Online TPP Program

#Question id: 39289

#Plant Biotechnology

Q. Assertion:Brassica napus lines carrying Ogura (radish) cytoplasm are highly efficient for commercial growth in cold climates without any modifications. 

Reason: The presence of radish chloroplasts in these lines causes chlorophyll deficiency under low temperatures.

TLS Online TPP Program

#Question id: 39290

#Plant Biotechnology

Q. Assertion: Protoplast fusion between CMS B. napus (with radish cytoplasm) and normal B. napus can produce healthy male sterile plants. 

Reason: The resulting cybrids can retain the radish mitochondria (for CMS) while replacing the radish chloroplasts with B. napus chloroplasts.

TLS Online TPP Program

#Question id: 39291

#Plant Biotechnology

Q. Assertion: In plant breeding, cytoplasm transfer via somatic hybridization is significantly faster than conventional backcrossing. 

Reason: Protoplast fusion can achieve in one year what typically takes 5–6 years through the backcrossing method.

TLS Online TPP Program

#Question id: 39292

#Plant Biotechnology

Q. Assertion: Symmetric hybrids allow for the transfer of genes between species that are sexually incompatible. 

Reason: Somatic hybridization bypasses the requirements of pollination and fertilization, enabling fusion between even completely sterile lines like monoploids.

TLS Online TPP Program

#Question id: 39293

#Plant Biotechnology

Q. Assertion: Cybridization enables the creation of unique organelle combinations that cannot be produced through sexual reproduction. 

Reason: Somatic fusion allows the mitochondria of one species to be combined with the chloroplasts of another, often resulting in recombinant organellar genomes.