Choose the correct order for the steps involve in Insitu hybridization.

1. Cell permeaabilisation

2. Detection of hybridised probe radioactive.

3. Wash excess probe from section

4. Cell fixation

TLS Online TPP Program

#Question id: 38736

#Plant Biotechnology

Q. Regarding callus formation from protoplasts:

1. Callus formation requires prior regeneration of the cell wall.

2. Callus cells are genetically identical to the donor plant.

3. Callus represents a dedifferentiated state.

4. High auxin concentration promotes callus induction.

Which statements are correct?

TLS Online TPP Program

#Question id: 38739

#Plant Biotechnology

Q. Which of the following pairs is INCORRECTLY matched?

TLS Online TPP Program

#Question id: 38740

#Plant Biotechnology

Q. Identify the correctly matched set used commonly for plant protoplast isolation:

TLS Online TPP Program

#Question id: 38741

#Plant Biotechnology

Q. Which enzyme combination is MOST appropriate for complete degradation of primary plant cell wall during protoplast isolation?

TLS Online TPP Program

#Question id: 38742

#Plant Biotechnology

Q: Which of the following combinations of statements is entirely CORRECT?

I. Cell wall components include cellulose, hemicellulose, and pectin.

II. The optimal temperature range for isolation is 25-30°C.

III. Barley aleurone cells are treated with Zymolyase to form sphaeroplasts.

IV. The one-step method involves using an enzyme mixture together simultaneously.

TLS Online TPP Program

#Question id: 38744

#Plant Biotechnology

Match the following enzymes commonly used in plant tissue maceration with their sources:

List I	List II
A. Zymolyase	1. Rhizopus arrhizus
B. Pectolyase	2. Arthrobacter luteus
C. Macerase	3. Aspergillus japonicus
D. Macerozyme R-10	4. Helix pomatia

Select the correct option: