TLS Online TPP Program

#Question id: 24217


Choose the correct statement of the following
a. In general, high acetyl-CoA or citrate are present, or when a high proportion of the cell’s adenylate is in the form of ATP, gluconeogenesis is favored.
b. When the level of AMP increases, it promotes glycolysis by stimulating PFK-1 by inhibiting FBPase-1
c. Phosphorylation of fructose 6-phosphate, catalysed by phosphofructokinase-2 (PFK-2)
d. Glucagon increases the cellular level of fructose 2,6 bisphosphate, stimulating glycolysis and inhibiting gluconeogenesis.

#Biochemistry
  1. a & b only
  2. b & d only
  3. a, b & c
  4. a, b, c, & d
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TLS Online TPP Program

#Question id: 40211

#Environmental Science

 Match the scientists in List I with their proposed theories or experiments in List II.

List I (Scientists)List II (Theories/Experiments)
A. A.I. Oparin & J.B.S. HaldaneI. Swan-necked flask experiment (Biogenesis)
B. S.L. Miller & H.C. UreyII. Coacervate droplets (Chemical Evolution)
C. Louis PasteurIII. Microspheres (Thermal synthesis of proteins)
D. Sidney FoxIV. Simulation of primitive earth conditions

TLS Online TPP Program

#Question id: 40212

#Applied Microbiology

Read the following statements regarding the steps of a standard PCR cycle and choose the correct option.

Statement I: The denaturation step involves the breaking of phosphodiester bonds to separate the double-stranded DNA into single strands.

Statement II: The annealing temperature is usually set 3 -5 0   C lower than the melting temperature (Tm) of the primers to ensure specific binding.

TLS Online TPP Program

#Question id: 40213

#Applied Microbiology

In the following question, a statement of Assertion (A) is followed by a statement of Reason (R).

Assertion (A): The annealing step must be performed at a significantly lower temperature than the denaturation step.

Reason (R): High temperatures destabilize hydrogen bonds, while lower temperatures favor the formation of hydrogen bonds required for primers to hybridize with the template.

TLS Online TPP Program

#Question id: 40214

#Applied Microbiology

Which of the following represents the correct sequence of temperature changes during one single cycle of PCR amplification? 

TLS Online TPP Program

#Question id: 40215

#Environmental Science

Match the geological eons/events in List I with the biological milestones in List II.

List I (Time Period/Event)List II (Biological Milestone)
A. 4.0 - 3.8 Billion years agoI. Appearance of first Eukaryotes
B. 3.5 Billion years agoII. Oxygen Revolution (Great Oxidation Event)
C. 2.7 - 2.5 Billion years agoIII. Appearance of first cellular life (Prokaryotes)
D. 2.0 - 1.5 Billion years agoIV. Formation of life precursors (RNA/Protocells)

TLS Online TPP Program

#Question id: 40216

#Environmental Science

Given below are two statements regarding the Miller-Urey experiment:
Statement I: The experiment used a mixture of Methane (CH4), Ammonia (NH3), Hydrogen (H2), and Water vapor (H2O) exposed to electric discharge.
Statement II: The experiment successfully synthesized complex nucleic acids (DNA chains) within the first week.
In light of the above statements, choose the most appropriate answer: