Column 1	Column 2
(a) the electron donor	1. NADH
(b) the electron acceptor	2. E-FMN
(c) the conjugate redox pair	3. NAD+/NADH
(d) the reducing agent
(e) the oxidizing agent

Match the following 

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#Question id: 40428

#Applied Microbiology

Which of the following stationary phases is most appropriate for the purification of a recombinant protein tagged with a Hexa-histidine (His-tag) sequence?

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#Question id: 40429

#Applied Microbiology

Read the following statements regarding chromatography techniques:

Statement I: Ion exchange chromatography separates molecules based on differences in their net surface charge.

Statement II: Gel filtration chromatography (Size Exclusion) separates molecules based on their specific binding to a ligand.

Choose the correct answer from the options given below:

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#Question id: 40430

#Applied Microbiology

Assertion (A): Total cellular RNA passed through an oligo(dT) column results in the specific elution of mRNA.

Reason (R): Prokaryotic rRNA and tRNA constitute the majority of total RNA and they lack a 3' Poly-A tail.

Choose the correct option:

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#Question id: 40431

#Applied Microbiology

Match the Chromatography Type (List I) with its Principle of Separation (List II).

List I (Chromatography)	List II (Basis of Separation)
(a) Ion Exchange	(i) Hydrophobicity
(b) Gel Filtration	(ii) Specific Ligand Interaction
(c) Affinity	(iii) Net Charge
(d) Reverse Phase	(iv) Molecular Size / Stokes Radius

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#Question id: 40432

#Botany

Match the enzymes in LIST-I with their specific metallic activators/co-factors in LIST-II.

LIST-I (Enzyme)	LIST-II (Activator/Co-factor)
A. Nitrogenase	I. Magnesium (Mg²⁺)
B. RuBisCO	II. Molybdenum (Mo)
C. Alcohol Dehydrogenase	III. Iron (Fe)
D. Ferredoxin	IV. Zinc (Zn²⁺)

Choose the correct answer from the options given below:

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#Question id: 40433

#Botany

Which of the following statements are correct regarding the C4 pathway of photosynthesis?

A. The primary CO₂ acceptor is a 3-carbon molecule, Phosphoenolpyruvate (PEP). 

B. The first stable product is a 4-carbon compound, Oxaloacetic Acid (OAA). 

C. The enzyme RuBisCO is absent in the mesophyll cells of C4 plants.

D. The decarboxylation of malic acid to release CO₂ occurs in the mesophyll cells.

Choose the correct answer from the options given below: