TLS Online TPP Program
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TLS Online TPP Program
#Question id: 37926
#Plant Biotechnology
Arrange the following steps involved in enzymatic isolation, culture, and regeneration of plant protoplasts in the correct sequence:
A. Enzymatic digestion of cell wall using cellulase and pectinase
B. Culturing of isolated protoplasts by hanging drop / feeder layer / agar methods
C. Surface sterilization and epidermis removal from leaf explant
D. Density gradient centrifugation to separate viable protoplasts
E. Callus formation followed by regeneration of a complete plant
Choose the correct order:
TLS Online TPP Program
#Question id: 37927
#Plant Biotechnology
Protoplasts require osmotic stabilizers in culture media because they:
TLS Online TPP Program
#Question id: 37928
#Plant Biotechnology
Which of the following is a non-ionic osmotic stabilizer commonly used in protoplast culture?
TLS Online TPP Program
#Question id: 37929
#Plant Biotechnology
Mannitol is preferred for leaf mesophyll protoplasts because it is:
TLS Online TPP Program
#Question id: 37930
#Plant Biotechnology
The commonly used concentration range of sugar solutions for osmotic stabilization of protoplasts is:
TLS Online TPP Program
#Question id: 37931
#Plant Biotechnology
Which of the following solutes is considered a metabolizable osmotic stabilizer?