Q.  Assertion: The enzymatic method is superior to the mechanical method for large-scale experiments. 

Reason: Enzymatic isolation provides high yield and good viability of protoplasts.

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#Question id: 38660

#Plant Biotechnology

Q. Why are leaves generally preferred for isolating protoplasts via the enzymatic method? 

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#Question id: 38663

#Plant Biotechnology

Q. When using callus as a source for protoplast isolation, which physiological condition is required? 

TLS Online TPP Program

#Question id: 38665

#Plant Biotechnology

Q. What is the primary advantage of the enzymatic method over the mechanical method of protoplast isolation? 

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#Question id: 38710

#Plant Biotechnology

Q. Assertion: The mechanical method of protoplast isolation is generally not used for meristematic cells. 

Reason: Meristematic cells are less vacuolated, making them unsuitable for the plasmolysis-dissection principle

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#Question id: 38714

#Plant Biotechnology

Q. Assertion: Barley aleurone cells require treatment with glusulase to release protoplasts. 

Reason: Treatment of these cells with cellulase results in sphaeroplasts, which still possess a residual cell wall.

TLS Online TPP Program

#Question id: 38715

#Plant Biotechnology

Q. The enzymatic method of protoplast isolation, introduced by Cocking in 1960, primarily uses which enzyme for cell wall degradation?