Q. The enzymatic method of protoplast isolation, introduced by Cocking in 1960, primarily uses which enzyme for cell wall degradation? 

TLS Online TPP Program

#Question id: 37974

#Plant Biotechnology

Which of the following phenomena is primarily responsible for the wide variation in ploidy levels observed in plants regenerated from anther or microspore culture?

TLS Online TPP Program

#Question id: 37975

#Plant Biotechnology

In the context of determining ploidy in potato plants using stomatal guard cells, a chloroplast count of 18–24 per guard cell indicates that the plant is:

TLS Online TPP Program

#Question id: 37976

#Plant Biotechnology

Which staining agent is specifically recommended for determining ploidy by counting the number of nucleoli?

TLS Online TPP Program

#Question id: 37977

#Plant Biotechnology

List I (Method)	List II (Feature)
A. Flow Cytometry	1. Counts plastids in stomata
B. Chromosome Number	2. Uses Acetocarmine stain
C. Nucleoli Count	3. Measures Nuclear DNA content
D. Indirect Method	4. Distinguishes 1 vs 2 organelles

TLS Online TPP Program

#Question id: 37978

#Plant Biotechnology

Why is ploidy analysis considered an essential step after anther culture?

TLS Online TPP Program

#Question id: 37979

#Plant Biotechnology

Which of the following statements regarding the "Number of Nucleoli" method for ploidy determination is CORRECT?