Q.  Regarding density gradient centrifugation used in protoplast purification:

1. Separation is based on buoyant density.

2. Viable protoplasts form a distinct band.

3. Broken cells sediment faster than intact protoplasts.

4. The method separates cells based on size alone.

Which statements are correct?

TLS Online TPP Program

#Question id: 24216

#Biochemistry

In general, high acetyl-CoA or citrate are present, or when a high proportion of the cell’s adenylate is in the form of ATP then

TLS Online TPP Program

#Question id: 24217

#Biochemistry

Choose the correct statement of the following

a. In general, high acetyl-CoA or citrate are present, or when a high proportion of the cell’s adenylate is in the form of ATP, gluconeogenesis is favored.

b. When the level of AMP increases, it promotes glycolysis by stimulating PFK-1 by inhibiting FBPase-1

c. Phosphorylation of fructose 6-phosphate, catalysed by phosphofructokinase-2 (PFK-2)

d. Glucagon increases the cellular level of fructose 2,6 bisphosphate, stimulating glycolysis and inhibiting gluconeogenesis.

TLS Online TPP Program

#Question id: 24218

#Biochemistry

Phosphorylation of fructose 6-phosphate, catalysed by

TLS Online TPP Program

#Question id: 24219

#Biochemistry

When Fructose 2,6-bisphosphate binds to its allosteric site on PFK-1 results

TLS Online TPP Program

#Question id: 24219

#Applied Microbiology

When Fructose 2,6-bisphosphate binds to its allosteric site on PFK-1 results

TLS Online TPP Program

#Question id: 24220

#Biochemistry

Glucagon stimulates cAMP from ATP. Cyclic AMP then activates cAMP-dependent protein kinase, phosphorylates PFK-2/FBPase-2. Phosphorylation of this protein enhances its FBPase-2 activity and inhibits its PFK-2 activity resulting