Q. Assertion (A): Co-culturing is a vital technique for the regeneration of recalcitrant protoplasts. 

Reason (R): Fast-growing protoplasts in the medium secrete diffusible substances that stimulate cell wall synthesis in neighboring cells.

TLS Online TPP Program

#Question id: 38735

#Plant Biotechnology

Q. With reference to feeder layer technique in protoplast culture:

1. Feeder cells provide diffusible growth factors.

2. It compensates for the absence of cell-to-cell signaling.

3. Feeder cells directly fuse with protoplasts.

4. It increases plating efficiency of isolated protoplasts.

Which statements are correct?

TLS Online TPP Program

#Question id: 38736

#Plant Biotechnology

Q. Regarding callus formation from protoplasts:

1. Callus formation requires prior regeneration of the cell wall.

2. Callus cells are genetically identical to the donor plant.

3. Callus represents a dedifferentiated state.

4. High auxin concentration promotes callus induction.

Which statements are correct?

TLS Online TPP Program

#Question id: 38739

#Plant Biotechnology

Q. Which of the following pairs is INCORRECTLY matched?

TLS Online TPP Program

#Question id: 38740

#Plant Biotechnology

Q. Identify the correctly matched set used commonly for plant protoplast isolation:

TLS Online TPP Program

#Question id: 38741

#Plant Biotechnology

Q. Which enzyme combination is MOST appropriate for complete degradation of primary plant cell wall during protoplast isolation?

TLS Online TPP Program

#Question id: 38742

#Plant Biotechnology

Q: Which of the following combinations of statements is entirely CORRECT?

I. Cell wall components include cellulose, hemicellulose, and pectin.

II. The optimal temperature range for isolation is 25-30°C.

III. Barley aleurone cells are treated with Zymolyase to form sphaeroplasts.

IV. The one-step method involves using an enzyme mixture together simultaneously.