TLS Online TPP Program

#Question id: 38756


Q. Why does FDA (Fluorescein diacetate) only accumulate in viable protoplasts?

#Plant Biotechnology
  1. It only binds to the cellulose in the cell wall. 
  2. It requires an intact plasma membrane to be retained and esterase activity to become fluorescent. 
  3. It is a toxic dye that only dead cells can neutralize. 
  4. It is specifically designed to stain chloroplasts