TLS Online TPP Program

#Question id: 38807


Q. Why might a plant that survives thermotherapy be at a disadvantage later on?

#Plant Biotechnology
  1.  It becomes genetically modified and cannot reproduce. 
  2. Its defense mechanisms may have been inactivated by the heat. 
  3. It grows too quickly for the soil to provide adequate nutrients. 
  4. It loses its ability to perform photosynthesis at normal temperatures.

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TLS Online TPP Program

#Question id: 38764

#Plant Biotechnology

Q. What is the primary cause of membrane "disturbance" during the PEG-induced fusion process? 

TLS Online TPP Program

#Question id: 38765

#Plant Biotechnology

Q. Why is the "washing process" considered a critical step for actual fusion to occur?


TLS Online TPP Program

#Question id: 38766

#Plant Biotechnology

Q. Statement 1 (Assertion): Protoplasts located in close proximity are more likely to undergo fusion during the washing phase of a PEG treatment. 
Statement 2 (Reason): The removal of PEG molecules disrupts the organized structure of the plasma lemma, creating localized instabilities that facilitate membrane merging
Which of the following is correct? 

TLS Online TPP Program

#Question id: 38767

#Plant Biotechnology

Q.  Statement I: Polyethylene glycol (PEG) facilitates protoplast fusion by disturbing the plasma membrane organization.
Statement II: PEG can bind Ca²⁺ ions, which act as bridges between negatively charged PEG and plasma membrane components.

TLS Online TPP Program

#Question id: 38768

#Plant Biotechnology

Q. Statement I: PEG is negatively charged and can interact with cations such as Ca²⁺.
Statement II: Ca²⁺ ions can bind to negatively charged molecules on the plasma membrane, promoting membrane proximity.

TLS Online TPP Program

#Question id: 38769

#Plant Biotechnology

Q. Statement I: During the washing step, PEG may remove plasma membrane components bound to it.
Statement II: Removal of these components can destabilize membrane integrity and promote fusion of adjacent protoplasts.