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TLS Online TPP Program

#Question id: 921

Which of the following statements concerning the B oxidation of fatty acids is true?

#Applied Microbiology

  1. About 1,200 ATP molecules are ultimately produced per 20-carbon fatty acid oxidized.

  2. One FADH2 and two NADH are produced for each acetyl-CoA.

  3. The free fatty acid must be carboxylated in the B position by a biotin-dependent reaction before the process of B oxidation commences.

  4. The free fatty acid must be converted to a thioester before the process of B oxidation commences.

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TLS Online TPP Program

#Question id: 40198

#Applied Microbiology

Read the following statements regarding Fed-Batch culture and choose the correct option.

Statement I: Fed-batch culture is ideal for producing secondary metabolites (like antibiotics) because it allows the culture to be maintained in the stationary phase for a prolonged period.

Statement II: In fed-batch culture, the volume of the culture broth remains constant throughout the fermentation process. 
TLS Online TPP Program

#Question id: 40199

#Applied Microbiology

Match the parameter in Column A with its formula or definition in Column B

Column A (Parameter)Column B (Definition/Formula)
1. Dilution Rate (D)A. ln (2) / mu
2. Doubling Time (td)B. Flow rate (F) / Volume (V)
3. Yield Coefficient (Y)C. Change in cell mass / Change in substrate consumed
4. Specific Growth Rate (mu)D. 1/x . dx/dt
TLS Online TPP Program

#Question id: 40200

#Applied Microbiology

Calculate the melting temperature (Tm) for the following primer sequence using the Wallace Rule

Sequence: 5'-GGCCAAAATTTTGGCC-3'
TLS Online TPP Program

#Question id: 40201

#Environmental Science

Match List I (Atmospheric Layers) with List II (Characteristics):

List I (Layer)List II (Characteristic)
A. TroposphereI. Reflects radio waves
B. StratosphereII. Lapse rate is positive (Temperature decreases with height)
C. ThermosphereIII. Contains the Ozone layer
D. IonosphereIV. Temperature increases significantly with height due to solar radiation
TLS Online TPP Program

#Question id: 40202

#Applied Microbiology

Assertion (A): Primers with high GC content generally have a higher melting temperature (Tm) than AT-rich primers of the same length.

Reason (R): Guanine and Cytosine are bonded by three hydrogen bonds, which require more energy to break than the two hydrogen bonds between Adenine and Thymine
TLS Online TPP Program

#Question id: 40203

#Applied Microbiology

Which cofactor is essential for the activity of Taq DNA polymerase during the PCR extension step?