TLS Online TPP Program
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TLS Online TPP Program
#Question id: 38742
#Plant Biotechnology
Q: Which of the following combinations of statements is entirely CORRECT?
I. Cell wall components include cellulose, hemicellulose, and pectin.
II. The optimal temperature range for isolation is 25-30°C.
III. Barley aleurone cells are treated with Zymolyase to form sphaeroplasts.
IV. The one-step method involves using an enzyme mixture together simultaneously.
TLS Online TPP Program
#Question id: 38744
#Plant Biotechnology
Match the following enzymes commonly used in plant tissue maceration with their sources:
| List I | List II |
| A. Zymolyase | 1. Rhizopus arrhizus |
| B. Pectolyase | 2. Arthrobacter luteus |
| C. Macerase | 3. Aspergillus japonicus |
| D. Macerozyme R-10 | 4. Helix pomatia |
TLS Online TPP Program
#Question id: 38745
#Plant Biotechnology
Q: Assertion (A): An osmoticum must be added to the medium during the isolation of protoplasts.
Reason (R): Protoplasts lack a cell wall and are prone to bursting without osmotic stabilization.
TLS Online TPP Program
#Question id: 38747
#Plant Biotechnology
Q. Assertion (A): Co-culturing is a vital technique for the regeneration of recalcitrant protoplasts.
Reason (R): Fast-growing protoplasts in the medium secrete diffusible substances that stimulate cell wall synthesis in neighboring cells.
TLS Online TPP Program
#Question id: 38748
#Plant Biotechnology
Q. Assertion (A): In a co-culture system, the two types of protoplasts must be in direct physical contact to exchange nutrients.
Reason (R): Growth factors provided by "nurse" cells are diffusible and can move through the liquid or semi-solid culture medium.
TLS Online TPP Program
#Question id: 38749
#Plant Biotechnology
Q. Assertion (A): Protoplasts are initially fragile and cannot undergo mitosis immediately after isolation.
Reason (R): The enzymatic degradation of the cell wall during isolation removes the structural constraint required for organized cell division.