Practice Questions

TLS Online TPP Program

#Question id: 39226

Q. Most somaclonal variants are expressed in the R₁ generation rather than R₀ because:

#Plant Biotechnology

Tissue culture does not induce mutations in R₀.
Mutations in R₀ are often heterozygous and require selfing to become homozygous.
R₀ plants are genetically identical to the donor plant.
R₁ plants are grown under stress conditions.

More Questions

TLS Online TPP Program

#Question id: 37921

#Plant Biotechnology

The mechanical method of protoplast isolation is best suited for which type of tissues?

TLS Online TPP Program

#Question id: 37922

#Plant Biotechnology

Which of the following plant materials is commonly used for mechanical isolation of protoplasts?

TLS Online TPP Program

#Question id: 37923

#Plant Biotechnology

The basic principle of mechanical protoplast isolation involves:

TLS Online TPP Program

#Question id: 37924

#Plant Biotechnology

Which of the following statements about the mechanical method of protoplast isolation is INCORRECT?

TLS Online TPP Program

#Question id: 37925

#Plant Biotechnology

Which type of cells is NOT suitable for protoplast isolation by the mechanical method?

TLS Online TPP Program

#Question id: 37926

#Plant Biotechnology

Arrange the following steps involved in enzymatic isolation, culture, and regeneration of plant protoplasts in the correct sequence:

A. Enzymatic digestion of cell wall using cellulase and pectinase

B. Culturing of isolated protoplasts by hanging drop / feeder layer / agar methods

C. Surface sterilization and epidermis removal from leaf explant

D. Density gradient centrifugation to separate viable protoplasts

E. Callus formation followed by regeneration of a complete plant

Choose the correct order: