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TLS Online TPP Program

#Question id: 39260

Q. Assertion (A): All asymmetric hybrids contained nitrate reductase gene and rDNA repeats of Atropa belladonna.
Reason (R): rDNA repeats are often preferentially retained during asymmetric hybridization.

#Plant Biotechnology
  1.  Both A and R are true and R correctly explains A
  2. Both A and R are true but R does not explain A
  3. A is true but R is false
  4. A is false but R is true
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TLS Online TPP Program

#Question id: 38744

#Plant Biotechnology

Match the following enzymes commonly used in plant tissue maceration with their sources:

List IList II
A. Zymolyase1. Rhizopus arrhizus
B. Pectolyase2. Arthrobacter luteus
C. Macerase3. Aspergillus japonicus
D. Macerozyme R-104. Helix pomatia
Select the correct option:
TLS Online TPP Program

#Question id: 38745

#Plant Biotechnology

Q: Assertion (A): An osmoticum must be added to the medium during the isolation of protoplasts.
Reason (R): Protoplasts lack a cell wall and are prone to bursting without osmotic stabilization.
TLS Online TPP Program

#Question id: 38747

#Plant Biotechnology

Q. Assertion (A): Co-culturing is a vital technique for the regeneration of recalcitrant protoplasts. 
Reason (R): Fast-growing protoplasts in the medium secrete diffusible substances that stimulate cell wall synthesis in neighboring cells.
TLS Online TPP Program

#Question id: 38748

#Plant Biotechnology

Q. Assertion (A): In a co-culture system, the two types of protoplasts must be in direct physical contact to exchange nutrients.
 Reason (R): Growth factors provided by "nurse" cells are diffusible and can move through the liquid or semi-solid culture medium.
TLS Online TPP Program

#Question id: 38749

#Plant Biotechnology

Q. Assertion (A): Protoplasts are initially fragile and cannot undergo mitosis immediately after isolation. 
Reason (R): The enzymatic degradation of the cell wall during isolation removes the structural constraint required for organized cell division.
TLS Online TPP Program

#Question id: 38751

#Plant Biotechnology

Q.Assertion (A): Fast-growing protoplasts act as a "feeder layer" in co-culture experiments. 
Reason (R): They compete with slow-growing protoplasts for limited carbon sources, thereby forcing the slow-growing cells to adapt faster.