Q. Assertion: Symmetric hybrids are utilized to widen the genetic base of allopolyploid species.

 Reason: Producing synthetic lines of Brassica napus by fusing B. oleracea and B. campestris introduces desirable traits that might be missing in the current cultivated pool.

TLS Online TPP Program

#Question id: 38772

#Plant Biotechnology

Q. Statement I: Protoplasts are isolated by enzymatic digestion of the cell wall using a combination of cellulase and pectinase.

Statement II: Cellulose microfibrils and pectic substances are major structural components of plant cell walls.

TLS Online TPP Program

#Question id: 38773

#Plant Biotechnology

Q.Statement I: The pH of the enzyme solution for protoplast isolation is maintained between 4.7 and 8.0.

Statement II: Enzymes involved in cell wall degradation show activity only at neutral pH.

TLS Online TPP Program

#Question id: 38774

#Plant Biotechnology

Q. Statement I: Protoplast isolation is usually carried out at a temperature range of 25–30°C.

Statement II: This temperature range supports optimal enzyme activity and maintains protoplast viability.

TLS Online TPP Program

#Question id: 38775

#Plant Biotechnology

Q. Statement I: High osmotic concentration of the enzyme mixture is maintained using sorbitol or mannitol.

Statement II: Elevated osmoticum prevents protoplast bursting by balancing internal turgor pressure.

TLS Online TPP Program

#Question id: 38776

#Plant Biotechnology

Q. Statement I: Calcium chloride is added to the osmoticum during protoplast isolation.

Statement II: Calcium ions improve plasma membrane stability of protoplasts.

TLS Online TPP Program

#Question id: 38777

#Plant Biotechnology

Q. Statement I: B5 and MS media are commonly used for culturing isolated protoplasts.

Statement II: These media provide essential macro- and micronutrients required for protoplast survival and division.