Q. Assertion:Brassica napus lines carrying Ogura (radish) cytoplasm are highly efficient for commercial growth in cold climates without any modifications. 

Reason: The presence of radish chloroplasts in these lines causes chlorophyll deficiency under low temperatures.

#Question id: 40426

#Applied Microbiology

Assertion (A): Shuttle vectors are naturally occurring plasmids found in Agrobacterium tumefaciens.

Reason (R): They contain two origins of replication, enabling them to replicate in two different host organisms

Choose the correct option: 

#Question id: 40427

#Applied Microbiology

Match the Vector Type (List I) with its unique Feature/Function (List II).

#Question id: 40428

#Applied Microbiology

Which of the following stationary phases is most appropriate for the purification of a recombinant protein tagged with a Hexa-histidine (His-tag) sequence?

#Question id: 40429

#Applied Microbiology

Read the following statements regarding chromatography techniques:

Statement I: Ion exchange chromatography separates molecules based on differences in their net surface charge.

Statement II: Gel filtration chromatography (Size Exclusion) separates molecules based on their specific binding to a ligand.

Choose the correct answer from the options given below:

#Question id: 40430

#Applied Microbiology

Assertion (A): Total cellular RNA passed through an oligo(dT) column results in the specific elution of mRNA.

Reason (R): Prokaryotic rRNA and tRNA constitute the majority of total RNA and they lack a 3' Poly-A tail.

Choose the correct option:

#Question id: 40431

#Applied Microbiology

Match the Chromatography Type (List I) with its Principle of Separation (List II).