Q. Assertion: Symmetric hybrids allow for the transfer of genes between species that are sexually incompatible. 

Reason: Somatic hybridization bypasses the requirements of pollination and fertilization, enabling fusion between even completely sterile lines like monoploids.

TLS Online TPP Program

#Question id: 39277

#Plant Biotechnology

Q. Which of the following traits is most likely to arise as a novel characteristic in somatic hybrids?

TLS Online TPP Program

#Question id: 39278

#Plant Biotechnology

Q. Consider the following statements regarding cytoplasm transfer via somatic hybridization:

Statement I: Cytoplasm transfer that normally takes 6–7 years via backcrossing can be achieved in just one year using protoplast fusion.

Statement II: Iodoacetate is used to inactivate the nucleus of the recipient parent protoplasts.

Statement III: Irradiation of donor protoplasts is used to inactivate their nuclei.

Statement IV: The resulting cybrids contain the nucleus of parent A and mitochondria of parent B.

Which of the statements are correct?

TLS Online TPP Program

#Question id: 39279

#Plant Biotechnology

Q. Regarding the tomato × Solanum acaule cybrids:

Statement I: Regenerants were morphologically and cytologically similar to tomato.

Statement II: They exhibited cytoplasmic male sterility (CMS) inherited maternally.

Statement III: CMS expression disappeared after one generation.

Statement IV: Some regenerants had malformed or nonfunctional pollen.

Which statements are correct?

TLS Online TPP Program

#Question id: 39280

#Plant Biotechnology

Q. Regarding cybridization for male sterile line development:

Statement I: Cybrids allow rapid transfer of mitochondria even across sexually incompatible species.

Statement II: Male sterile lines developed via protoplast fusion can be used for hybrid seed production using CMS restorers.

Statement III: Nuclear genomes are exchanged between donor and recipient protoplasts in cybridization.

Which statements are correct?

TLS Online TPP Program

#Question id: 39281

#Plant Biotechnology

Q. Statement I: Conventional backcrossing is a more time-efficient method for cytoplasm transfer compared to somatic hybridization. 

Statement II: Protoplast fusion can achieve cytoplasm transfer in approximately one year.

TLS Online TPP Program

#Question id: 39282

#Plant Biotechnology

Q. Statement I: In the cybrid approach, iodoacetate is used to inactivate the nuclei of the recipient strain (Parent A). 

Statement II: Irradiation is used to inactivate the nuclei of the donor strain (Parent B) to ensure only its cytoplasm is transferred.