#Question id: 40221
#Applied Microbiology
A gene incorporated into a vector that confers a trait suitable for artificial selection of transformed cells (e.g., survival on ampicillin-containing agar) is best known as a:
#Question id: 40222
#Applied Microbiology
many cloning vectors, an antibiotic resistance gene is used for ________, while a gene like lacZ (for blue-white color change) is often used for ________.
#Question id: 40223
#Applied Microbiology
An E. coli cell that successfully takes up a pBR322 plasmid (which carries ampicillin and tetracycline resistance genes) will be able to grow on:
#Question id: 40224
#Applied Microbiology
Match the vector component in Column A with its primary function in Column B
| Column A (Component) | Column B (Function) |
| 1. ori site | a. Allows for the insertion of foreign DNA using restriction enzymes. |
| 2. Antibiotic resistance gene | b. Sequence responsible for initiating autonomous replication of the vector. |
| 3. Multiple Cloning Site (MCS) | c. Enables the identification and survival of transformed host cells under specific conditions. |
#Question id: 40225
#Applied Microbiology
Read the following statements regarding cloning vectors and select the correct option.
Statement I: Antibiotic resistance markers are essential for an expression vector but not necessary for a standard cloning vector.
Statement II: The presence of an antibiotic resistance gene allows transformed bacteria to survive on media containing the corresponding antibiotic.
#Question id: 40226
#Applied Microbiology
Assertion (A): When plating bacteria after a transformation experiment, adding ampicillin to the agar plates is crucial if the plasmid vector carries the ampR gene.
Reason (R): Ampicillin acts as a nutrient source for the bacteria that have successfully taken up the plasmid.