Practice Questions

TLS Online TPP Program

#Question id: 547

The role of an enzyme in an enzyme-catalyzed reaction is to:

#Biochemistry

bind a transition state intermediate, such that it cannot be converted back to substrate.
ensure that all of the substrate is converted to product.
ensure that the product is more stable than the substrate.
increase the rate at which substrate is converted into product.

More Questions

TLS Online TPP Program

#Question id: 38773

#Plant Biotechnology

Q.Statement I: The pH of the enzyme solution for protoplast isolation is maintained between 4.7 and 8.0.

Statement II: Enzymes involved in cell wall degradation show activity only at neutral pH.

TLS Online TPP Program

#Question id: 38774

#Plant Biotechnology

Q. Statement I: Protoplast isolation is usually carried out at a temperature range of 25–30°C.

Statement II: This temperature range supports optimal enzyme activity and maintains protoplast viability.

TLS Online TPP Program

#Question id: 38775

#Plant Biotechnology

Q. Statement I: High osmotic concentration of the enzyme mixture is maintained using sorbitol or mannitol.

Statement II: Elevated osmoticum prevents protoplast bursting by balancing internal turgor pressure.

TLS Online TPP Program

#Question id: 38776

#Plant Biotechnology

Q. Statement I: Calcium chloride is added to the osmoticum during protoplast isolation.

Statement II: Calcium ions improve plasma membrane stability of protoplasts.

TLS Online TPP Program

#Question id: 38777

#Plant Biotechnology

Q. Statement I: B5 and MS media are commonly used for culturing isolated protoplasts.

Statement II: These media provide essential macro- and micronutrients required for protoplast survival and division.

TLS Online TPP Program

#Question id: 38778

#Plant Biotechnology

Q. Statement I: Mannitol and sorbitol are preferred osmotic stabilizers during protoplast isolation.

Statement II: These compounds are metabolized rapidly by protoplasts to generate energy.