Phenyl-methane-sulfonyl-fluoride (PMSF) inactivates serine proteases by binding covalently to the catalytic serine residue at the active site; this enzyme-inhibitor bond is not cleaved by the enzyme.  This is an example of what kind of inhibition?

TLS Online TPP Program

#Question id: 38728

#Plant Biotechnology

Q. Totipotency of plant protoplasts is BEST demonstrated by their ability to

TLS Online TPP Program

#Question id: 38729

#Plant Biotechnology

Q. If a regenerated plant from protoplast culture shows unexpected phenotypic variation, the MOST plausible cause is

TLS Online TPP Program

#Question id: 38730

#Plant Biotechnology

Q. Which combination of conditions would MOST favor stable callus formation from mesophyll protoplasts?

TLS Online TPP Program

#Question id: 38731

#Plant Biotechnology

Q.Regarding epidermis removal during leaf preparation:

1. It improves penetration of cell wall–degrading enzymes.

2. It removes cells with lower regenerative capacity.

3. It prevents plasmolysis during enzyme treatment.

4. It exposes metabolically active mesophyll cells.

Which of the above statements are correct?

TLS Online TPP Program

#Question id: 38732

#Plant Biotechnology

Q. With reference to plasmolysis prior to enzymatic digestion:

1. It facilitates separation of plasma membrane from cell wall.

2. It reduces mechanical damage during wall digestion.

3. It is induced by placing tissues in hypotonic solution.

4.  It enhances enzyme accessibility to cellulose microfibrils.

Which statements are correct?

TLS Online TPP Program

#Question id: 38733

#Plant Biotechnology

Q. Concerning enzymatic isolation of protoplasts:

1. Cellulase hydrolyzes β-1,4-glycosidic bonds of cellulose.

2. Pectinase degrades the middle lamella.

3. Excessive enzyme exposure leads to osmotic bursting.

4. Enzyme treatment preserves plasmodesmata.

Which statements are correct?