Assertion (A): PCR has wide applications in biotechnology and diagnostics.
Reason (R): PCR allows exponential amplification of DNA in vitro.

TLS Online TPP Program

#Question id: 38741

#Plant Biotechnology

Q. Which enzyme combination is MOST appropriate for complete degradation of primary plant cell wall during protoplast isolation?

TLS Online TPP Program

#Question id: 38742

#Plant Biotechnology

Q: Which of the following combinations of statements is entirely CORRECT?

I. Cell wall components include cellulose, hemicellulose, and pectin.

II. The optimal temperature range for isolation is 25-30°C.

III. Barley aleurone cells are treated with Zymolyase to form sphaeroplasts.

IV. The one-step method involves using an enzyme mixture together simultaneously.

TLS Online TPP Program

#Question id: 38744

#Plant Biotechnology

Match the following enzymes commonly used in plant tissue maceration with their sources:

List I	List II
A. Zymolyase	1. Rhizopus arrhizus
B. Pectolyase	2. Arthrobacter luteus
C. Macerase	3. Aspergillus japonicus
D. Macerozyme R-10	4. Helix pomatia

Select the correct option:

TLS Online TPP Program

#Question id: 38745

#Plant Biotechnology

Q: Assertion (A): An osmoticum must be added to the medium during the isolation of protoplasts.

Reason (R): Protoplasts lack a cell wall and are prone to bursting without osmotic stabilization.

TLS Online TPP Program

#Question id: 38747

#Plant Biotechnology

Q. Assertion (A): Co-culturing is a vital technique for the regeneration of recalcitrant protoplasts. 

Reason (R): Fast-growing protoplasts in the medium secrete diffusible substances that stimulate cell wall synthesis in neighboring cells.

TLS Online TPP Program

#Question id: 38748

#Plant Biotechnology

Q. Assertion (A): In a co-culture system, the two types of protoplasts must be in direct physical contact to exchange nutrients.

 Reason (R): Growth factors provided by "nurse" cells are diffusible and can move through the liquid or semi-solid culture medium.