#Question id: 40425
#Applied Microbiology
Given below are two statements:
Statement I: A shuttle vector is designed to allow the cloning of a gene in E. coli and its subsequent expression in a eukaryotic cell.
Statement II: pBR322 is a classic example of a shuttle vector because it can replicate in both animals and bacteria.
In the light of the above statements, choose the correct answer from the options given below:
#Question id: 40426
#Applied Microbiology
Assertion (A): Shuttle vectors are naturally occurring plasmids found in Agrobacterium tumefaciens.
Reason (R): They contain two origins of replication, enabling them to replicate in two different host organisms
Choose the correct option:
#Question id: 40427
#Applied Microbiology
Match the Vector Type (List I) with its unique Feature/Function (List II).
| List I (Vector Type) | List II (Feature/Function) |
| (a) Expression Vector | (i) Can accomodate large DNA inserts (100-300 kb) |
| (b) Shuttle Vector | (ii) Designed for protein production (transcription/translation) |
| (c) BAC (Bacterial Artificial Chromosome) | (iii) Replicates in two unrelated host species |
| (d) Cosmid | (iv) Hybrid of plasmid and phage lambda 'cos' site |
#Question id: 40428
#Applied Microbiology
Which of the following stationary phases is most appropriate for the purification of a recombinant protein tagged with a Hexa-histidine (His-tag) sequence?
#Question id: 40429
#Applied Microbiology
Read the following statements regarding chromatography techniques:
Statement I: Ion exchange chromatography separates molecules based on differences in their net surface charge.
Statement II: Gel filtration chromatography (Size Exclusion) separates molecules based on their specific binding to a ligand.
Choose the correct answer from the options given below:
#Question id: 40430
#Applied Microbiology
Assertion (A): Total cellular RNA passed through an oligo(dT) column results in the specific elution of mRNA.
Reason (R): Prokaryotic rRNA and tRNA constitute the majority of total RNA and they lack a 3' Poly-A tail.
Choose the correct option: