TLS Online TPP Program
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TLS Online TPP Program
#Question id: 38710
#Plant Biotechnology
Q. Assertion: The mechanical method of protoplast isolation is generally not used for meristematic cells.
Reason: Meristematic cells are less vacuolated, making them unsuitable for the plasmolysis-dissection principle
TLS Online TPP Program
#Question id: 38713
#Plant Biotechnology
Q. Assertion: The enzymatic method is superior to the mechanical method for large-scale experiments.
Reason: Enzymatic isolation provides high yield and good viability of protoplasts.
TLS Online TPP Program
#Question id: 38714
#Plant Biotechnology
Q. Assertion: Barley aleurone cells require treatment with glusulase to release protoplasts.
Reason: Treatment of these cells with cellulase results in sphaeroplasts, which still possess a residual cell wall.
TLS Online TPP Program
#Question id: 38715
#Plant Biotechnology
Q. The enzymatic method of protoplast isolation, introduced by Cocking in 1960, primarily uses which enzyme for cell wall degradation?
TLS Online TPP Program
#Question id: 38716
#Plant Biotechnology
Q. Which physiological condition is most ideal for isolating protoplasts from callus tissue?
TLS Online TPP Program
#Question id: 38717
#Plant Biotechnology
Q. In the sequential (two-step) enzymatic isolation method, what is the correct order of enzyme treatment?
