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TLS Online TPP Program

#Question id: 40204

Match the PCR component in Column A with its specific function in Column B

Column A (Component)Column B (Function)
1. dNTPsA. Provides the starting point for DNA synthesis
2. PrimersB. Building blocks for the new DNA strand
3. BufferC. Maintains pH and salt conditions
4. Template DNAD. Contains the target region to be amplified

#Applied Microbiology
  1. 1-B, 2-A, 3-C, 4-D
  2. 1-A, 2-C, 3-B, 4-D
  3. 1-C, 2-B, 3-D, 4-A
  4.  1-B, 2-C, 3-A, 4-D
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TLS Online TPP Program

#Question id: 39259

#Plant Biotechnology

Q. Assertion (A): After repeated backcrossing of asymmetric hybrids with N. plumbaginifolia, plants with a diploid N. plumbaginifolia genome and a few A. belladonna chromosomes were recovered.
Reason (R): Backcrossing helps eliminate unwanted alien chromosomes while retaining selected genes.
TLS Online TPP Program

#Question id: 39260

#Plant Biotechnology

Q. Assertion (A): All asymmetric hybrids contained nitrate reductase gene and rDNA repeats of Atropa belladonna.
Reason (R): rDNA repeats are often preferentially retained during asymmetric hybridization.
TLS Online TPP Program

#Question id: 39261

#Plant Biotechnology

Q.Assertion (A): Hybrids obtained from irradiated carrot and non-irradiated rice protoplast fusion resembled rice plants.
Reason (R): The non-irradiated parent usually contributes more intact genomic material.
TLS Online TPP Program

#Question id: 39262

#Plant Biotechnology

Q. Assertion: The fusion of B. napus (AACC) and B. nigra (BB) results in a hybrid with the genomic constitution AABBCC. 
Reason: This specific interspecific fusion was primarily conducted to transfer resistance against Phoma lingam (black-leg disease) to rapeseed.
 
TLS Online TPP Program

#Question id: 39263

#Plant Biotechnology

Q. Assertion: Somatic hybridization between non-flowering and male sterile dihaploid clones of potato can result in male fertile hybrids. 
Reason: Symmetric hybridization allows for the creation of superior hybrids and the generation of novel genetic material by bypassing traditional sexual reproduction barriers.
TLS Online TPP Program

#Question id: 39264

#Plant Biotechnology

Q. Assertion: Tobacco hybrids produced from N. rustica and N. tabacum may exhibit resistance to blue mould. 
Reason: Blue mould resistance was a primary trait present in both parent lines and was intentionally selected for during the initial fusion process.