Practice Questions

TLS Online TPP Program

#Question id: 40210

Match the PCR step in Column I with the correct temperature range or characteristic in Column II.
Column I (PCR Step) Column II (Condition / Event)
A. Denaturation 1. ~72 °C; Optimum for Taq polymerase activity
B. Annealing 2. ~94 °C; Hydrogen bonds break to separate DNA strands
C. Extension 3. ~4 °C; Short-term storage after cycling
D. Final Hold 4. ~50–65 °C; Primers bind to the template

Column I (PCR Step)	Column II (Condition / Event)
A. Denaturation	1. ~72 °C; Optimum for Taq polymerase activity
B. Annealing	2. ~94 °C; Hydrogen bonds break to separate DNA strands
C. Extension	3. ~4 °C; Short-term storage after cycling
D. Final Hold	4. ~50–65 °C; Primers bind to the template