Match the resource with its environmental concern:

List I (Source)	List II (Impact/Issue)
A. Groundwater	3. Over-extraction (Depletion of aquifers)
B. Fossil fuel	2. Air pollution (Emissions from burning)
C. Soil	1. Salinization ( accumulation of salt in land)
D. Plastic waste	4. Microplastic contamination (Breakdown of plastics)

TLS Online TPP Program

#Question id: 38774

#Plant Biotechnology

Q. Statement I: Protoplast isolation is usually carried out at a temperature range of 25–30°C.

Statement II: This temperature range supports optimal enzyme activity and maintains protoplast viability.

TLS Online TPP Program

#Question id: 38775

#Plant Biotechnology

Q. Statement I: High osmotic concentration of the enzyme mixture is maintained using sorbitol or mannitol.

Statement II: Elevated osmoticum prevents protoplast bursting by balancing internal turgor pressure.

TLS Online TPP Program

#Question id: 38776

#Plant Biotechnology

Q. Statement I: Calcium chloride is added to the osmoticum during protoplast isolation.

Statement II: Calcium ions improve plasma membrane stability of protoplasts.

TLS Online TPP Program

#Question id: 38777

#Plant Biotechnology

Q. Statement I: B5 and MS media are commonly used for culturing isolated protoplasts.

Statement II: These media provide essential macro- and micronutrients required for protoplast survival and division.

TLS Online TPP Program

#Question id: 38778

#Plant Biotechnology

Q. Statement I: Mannitol and sorbitol are preferred osmotic stabilizers during protoplast isolation.

Statement II: These compounds are metabolized rapidly by protoplasts to generate energy.

TLS Online TPP Program

#Question id: 38779

#Plant Biotechnology

Q. Assertion (A): Vegetatively propagated crops are more prone to rapid spread of viral diseases.

Reason (R): Vegetative propagules directly transmit virus particles from the infected parent plant.

Choose the correct option: