TLS Online TPP Program

#Question id: 35


Arrange the following molecular components of an E. coli cell in terms of their percentage of total weight cell.

#Part-A Aptitude & General Biotechnology
  1. Water, Proteins, RNA, Polysaccharides

  2. Proteins, DNA, Polysaccharides, Lipids

  3. Water, Proteins, Lipids, Polysaccharides

  4. Nucleic acids, Proteins, Lipids, Polysaccharides

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TLS Online TPP Program

#Question id: 12718

#Part-B Specialized Branches in Biotechnology

primary disruptions could be signaling the plant that a change in environmental conditions has occurred and that it’s time to respond by altering existing pathways or by activating stress-response pathways. At least five different types of stress-sensing mechanisms can be distinguished:

                       

                          COLUMN I

 

 

                              COLUMN II

 

A) Physical sensing

 

 

i) usually results from the detection of by-products that accumulate in cells due to the uncoupling of enzymatic or electron transfer reactions, such as the accumulation of ROS during stress caused by too much light.

 

 

B) Biophysical sensing

 

 

ii) often involves the presence of specialized proteins that have evolved to sense a particular stress; for example, calcium channels that can sense changes in temperature and alter Ca2+ homeostasis.

 

 

C) Metabolic sensing

 

 

iii) refers to modifications of DNA or RNA structure that do not alter genetic sequences, such as the changes in chromatin that occur during temperature stress.

 

 

D) Biochemical sensing

 

 

iv) refers to the mechanical effects of stress on the plant or cell structure, for example, the contraction of the plasma membrane from the cell wall during drought stress.

 

 

E) Epigenetic sensing

 

 

v) might involve changes in protein structure or enzymatic activity, such as the inhibition of different enzymes during heat stress.

 

 

  
Match the correct sequence sensing mechanism during in stress;

TLS Online TPP Program

#Question id: 12719

#Part-B Specialized Branches in Biotechnology

Which of the following is a key enzyme in the proline biosynthetic pathway?

TLS Online TPP Program

#Question id: 12722

#Part-B Specialized Branches in Biotechnology

Eleven amino-acid consensus sequence motif TAQAAKEKAXE is repeated in the protein,  is the structural characteristics of which type of group of LEA protein?

TLS Online TPP Program

#Question id: 12723

#Part-B Specialized Branches in Biotechnology

GroEL, GroES is an example of the  prokaryotic cell, this HSP protein is belong to the which of the following HSP class?

TLS Online TPP Program

#Question id: 12724

#Part-B Specialized Branches in Biotechnology

In response to sudden, 5 to 10°C rises in temperature, plants produce a unique set of proteins referred to as heat shock proteins (HSPs). There are five classes of heat shock proteins found in plants such as;

       HSP class

 Examples (Arabidopsis / prokaryotic)

      Cellular location

 

I) HSP100

 

 

a) AtTCP-1 / GroEL, GroES

 

i) Cytosol

 

II) HSP90

 

 

b) Various AtHSP22, AtHSP20, AtHSP18.2, AtHSP17.6 / IBPA/B

 

ii) mitochondria

 

III) HSP70

 

 

c) AtHSP101 / ClpB, ClpA/C

 

iii) chloroplasts

 

IV) HSP60

 

 

d) AtHSP70 / DnaK

 

iv) endoplasmic reticulum

 

V) smHSP

 

 

e) AtHSP90 / HtpG

 

Match the following HSPs   with their correct location and the examples of the HSPs;


TLS Online TPP Program

#Question id: 12727

#Part-A Aptitude & General Biotechnology

Name the form of DNA which may be produced with a DNA sequence made up from alternating purine and pyrimidine nucleotides.