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TLS Online TPP Program

#Question id: 51

 If the equilibrium constant, Keq, for the reaction ATP → ADP + Pi is 2.22 × 105 M, calculate the standard free-energy change, ΔG°, for the synthesis of ATP from ADP and Pi at 25 °C.

#Part-A Aptitude & General Biotechnology

  1. −30.5 kJ/mol

  2. 30.5 kJ/mol

  3. −16.5 kJ/mol

  4. 45.5 kJ/mol

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TLS Online TPP Program

#Question id: 14217

#Part-B Specialized Branches in Biotechnology

In a microbial system how true yields are related to apparent growth yield? 
TLS Online TPP Program

#Question id: 14218

#Part-B Specialized Branches in Biotechnology

In CSTR system at steady rate  
TLS Online TPP Program

#Question id: 14218

#Part-A Aptitude & General Biotechnology

In CSTR system at steady rate  
TLS Online TPP Program

#Question id: 14221

#Part-B Specialized Branches in Biotechnology

E. coli have a maximum respiration rate, qO2max, of about 240-mg O2/g-dry wt-h. It is desired to achieve a cell mass of 20 g dry wt/l. The kLa is 120 h-1 in a 1000-l reactor (800 l working volume). A gas stream enriched in oxygen is used (i.e., 80% O2) which gives a value of C* = 28 mg/L. If oxygen becomes limiting, growth and respiration slow; for example,

                                     
   where CL is the dissolved oxygen concentration in the fermenter. What is CL when the cell mass is  at 20 g/l?
TLS Online TPP Program

#Question id: 14237

#Part-B Specialized Branches in Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What is the minimum mass-transfer coefficient necessary to sustain this culture if the reactor is sparged with air at approximately 1 atm pressure ?         
TLS Online TPP Program

#Question id: 14238

#Part-B Specialized Branches in Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What mass-transfer coefficient is required if pure oxygen is used instead of air?