TLS Online TPP Program

#Question id: 937


Glutamate dehydrogenase has different roles in different organisms. Which of these listed is mismatched?

#Part-A Aptitude & General Biotechnology
  1. E. coli (bacterium) - generates NH4+ + glutamate

  2. N. crassa (fungus) - aminates a-ketoglutarate

  3. mammals - degradation of amino acids and release of NH4+

  4. mammals - formation of glutamate

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TLS Online TPP Program

#Question id: 19790

#Part-A Aptitude & General Biotechnology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?
a) PCR denaturation temperature calculated by using Y = X (1+E)n
b) Size of the primer negatively affects the efficiency of PCR
c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.
d) Primer annealing of PCR at 550C for 1.5 min.
e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?
a) PCR denaturation temperature calculated by using Y = X (1+E)n
b) Size of the primer negatively affects the efficiency of PCR
c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.
d) Primer annealing of PCR at 550C for 1.5 min.
e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Part-A Aptitude & General Biotechnology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA PolymeraseSources
A- Tmai) Pyrococcus furiosus
B- Deep Vent™ii) Pyrococcus woesi
C- Tliiii) Thermotoga maritima
D- Pfuiv) Pyrococcus sp.
E- Pwov) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19840

#Part-A Aptitude & General Biotechnology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA PolymeraseSources
A- Tmai) Pyrococcus furiosus
B- Deep Vent™ii) Pyrococcus woesi
C- Tliiii) Thermotoga maritima
D- Pfuiv) Pyrococcus sp.
E- Pwov) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19841

#Part-A Aptitude & General Biotechnology

Which of the following is true regarding Reverse transcriptase ?