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TLS Online TPP Program

#Question id: 671

Which was a major scientific accomplishment by Linus Pauling?

#Part-A Aptitude & General Biotechnology

  1. Determined the α-helix structure in keratin.

  2. Discovered β-strands and described their assembly into sheets.

  3. Determined the three-dimensional structure of hemoglobin and myoglobin.

  4. Determined the structure of penicillin.

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TLS Online TPP Program

#Question id: 393

#Part-B Specialized Branches in Biotechnology

Carbon dioxide (CO2) is readily soluble in water, according to the equation CO2 + H2O ↔ H2CO3. Carbonic acid (H2CO3) is a weak acid. If CO2 is bubbled into a beaker containing pure, freshly distilled water, which of the following graphs correctly describes the results?
TLS Online TPP Program

#Question id: 15175

#Part-B Specialized Branches in Biotechnology

The cell membranes of psychrophilic microorganisms have high levels of _______ and remain semifluid when cold.
TLS Online TPP Program

#Question id: 14255

#Part-B Specialized Branches in Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?
TLS Online TPP Program

#Question id: 19787

#Part-A Aptitude & General Biotechnology

Polymerase chain reaction is based on___
a) Thermal modulation
b) pH dependent
c) Formation of amplicons by inserting some inputs for analysing its efficiency
d) Inter-complementary primers formation
TLS Online TPP Program

#Question id: 14238

#Part-B Specialized Branches in Biotechnology

A genetically-engineered strain of yeast is cultured in a bioreactor at 30⁰C for production of heterologous protein. The oxygen requirement is 80 mmol 1-1 h-I; the critical oxygen concentration is 0.004 mM. The solubility of oxygen in the fermentation broth is estimated to be 10% lower than in water due to solute effects. What mass-transfer coefficient is required if pure oxygen is used instead of air?