TLS Online TPP Program

#Question id: 5243


Auxotroph culture (His- and Lac-) culture of E. coli was mutagenised. On what media would one spread the mutagenised cells to select prototroph for both His+  and Lac+ cells?

#Part-A Aptitude & General Biotechnology
  1. Minimal media+Histidine

  2. Minimal media+Lactose

  3. Minimal media + Histidine + Lactose 

  4. Minimal media

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TLS Online TPP Program

#Question id: 11206

#Part-B Specialized Branches in Biotechnology

Tetrodotoxin blocks voltage-gated sodium channels, and ouabain blocks sodium-potassium pumps. If you added both tetrodotoxin and ouabain to a solution containing neural tissue, what responses would you expect?

TLS Online TPP Program

#Question id: 10892

#Part-B Specialized Branches in Biotechnology

Considering environment and ecosystem, identify the INCORRECT statement

TLS Online TPP Program

#Question id: 21009

#Part-B Specialized Branches in Biotechnology

Acetobacter aceti converts alcohol to acetic acid according to the stoichiometric  relation 
 C2H5OH + O2   →       CH3COOH + H2
In a vigorously agitated and aerated reactor containing 20 g/l ethanol, the organism  produces 16 g/l acetic acid and 2 g/l was the residual ethanol concentration. What are the  theoretical and observed yields of acetic acid expressed in g/g ethanol? 

TLS Online TPP Program

#Question id: 18942

#Part-A Aptitude & General Biotechnology

Bacterial artificial chromosomes (BACs), cosmids, phages, plasmids and yeast artificial chromosomes (YACs) are all commonly used cloning vectors that differ in their cloning capacities, with a range from approximately 100 bp to 1000 kb. Which of the following is the increasing cloning capacity?

TLS Online TPP Program

#Question id: 3667

#Part-A Aptitude & General Biotechnology

In an ______________ plasmid, replication occurs in two stages. In the first stage, the double stranded circular plasmid DNA replicates to form another double-stranded circular DNA and a single-stranded circular DNA, In the second stage, the complementary strand is synthesized on the single-stranded DNA to make another double-stranded DNA.