The immediate precursors of DNA and RNA synthesis in the cell all contain:

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#Question id: 13044

#Part-A Aptitude & General Biotechnology

How many different types of dNTPs are utilized in a reaction mixture when carrying out sanger’s DNA sequencing ?

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#Question id: 13045

#Part-A Aptitude & General Biotechnology

Maxam Gilbert DNA sequencing causes cleavage of DNA based on:

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#Question id: 13052

#Part-A Aptitude & General Biotechnology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

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#Question id: 13052

#Part-B Specialized Branches in Biotechnology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

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#Question id: 13053

#Part-A Aptitude & General Biotechnology

What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?

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#Question id: 13054

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?