Practice Questions

TLS Online TPP Program

#Question id: 5200

What is the difference between a linkage map and a physical map?

#Part-A Aptitude & General Biotechnology

For a linkage map, markers are spaced by recombination frequency, whereas for a physical map they are spaced by numbers of base pairs (bp).
For a physical map, the ATCG order and sequence must be achieved, but not for the linkage map.
For a linkage map, it is shown how each gene is linked to every other gene.
For a physical map, the distances must be calculable in units such as nanometers.

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TLS Online TPP Program

#Question id: 18774

#Part-A Aptitude & General Biotechnology

The above Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. In these plots, r equals moles of bound ligand/mole antibody and c is the concentration of free ligand, (Ka) is slope while intercept on x axis represent

TLS Online TPP Program

#Question id: 18775

#Part-A Aptitude & General Biotechnology

which of the following technique is a highly sensitive and quantitative procedure that utilizes radioactively labelled antigen or antibody

TLS Online TPP Program

#Question id: 18776

#Part-A Aptitude & General Biotechnology

In Western blotting, a protein mixture is separated by electrophoresis; then the protein bands are electrophoretically transferred onto nitrocellulose and identified with

TLS Online TPP Program

#Question id: 18777

#Part-A Aptitude & General Biotechnology

which of the following technique using antibodies labelled with fluorescent molecules can be used to visualize antigen on or within cells.

TLS Online TPP Program

#Question id: 18778

#Part-A Aptitude & General Biotechnology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

TLS Online TPP Program

#Question id: 18779

#Part-A Aptitude & General Biotechnology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?

Choose whether each of the following statements is true

a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence

b) Most antigens induce a polyclonal response.

c) For precipitation to occur, both antigen and antibody must be multivalent.

d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests