TLS Online TPP Program

#Question id: 2618


The three genes (lacZ, lacY, and lacA) are transcribed as a single mRNA from the promoter, these genes are expressed at high levels only when

#Part-A Aptitude & General Biotechnology
  1. Lactose is available

  2. Glucose, the preferred energy source is available

  3. Three regulatory proteins are involved.

  4. The Lac repressor is encoded by the lacI gene, which is located near the CAP Site.

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TLS Online TPP Program

#Question id: 18704

#Part-A Aptitude & General Biotechnology

If the weight of 1 mol bp is 660g What will the no. of moles in human genome having 3 x 109 bp

TLS Online TPP Program

#Question id: 18705

#Part-A Aptitude & General Biotechnology

What will weight in picogram in human genome 

TLS Online TPP Program

#Question id: 18706

#Part-A Aptitude & General Biotechnology

Sources of thermostable DNA polymerases with proofreading (3′–5′ exonuclease) activity are given match them correctly
DNA polymerase                          Source
a. Tma                                      i. Thermotoga maritima
b. Deep vent tm                      ii. Pyrococcus furiosus
c. Tli                                      iii. Thermococcus litoralis
d. Pfu                                      iv.  Pyrococcus sp
e. Pwo                                      v. Pyrococcus woesi

TLS Online TPP Program

#Question id: 18707

#Part-A Aptitude & General Biotechnology

primer dimers are formed when 

TLS Online TPP Program

#Question id: 18708

#Part-A Aptitude & General Biotechnology

PCR can be analysed amplification, Anneleaning, and extension and analysis is called

TLS Online TPP Program

#Question id: 18709

#Part-A Aptitude & General Biotechnology

qPCR can be done by 
i) cyanin dye or SYBR green – to measure amplification
ii) Taqman assay – measure extension
iii) molecular beacons - denaturation