Assuming that the average amino acid residue contributes 110 to the peptide molecular weight, what will be the minimum length of the mRNA encoding a protein of molecular weight 50,000?

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#Question id: 19790

#Part-A Aptitude & General Biotechnology

What will be the major difference between the PCR and replication is?

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#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19791

#Part-A Aptitude & General Biotechnology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Part-A Aptitude & General Biotechnology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep VentTM	ii) Pyrococcus woesi
C- Tli	iii)  Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19840

#Part-A Aptitude & General Biotechnology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep VentTM	ii) Pyrococcus woesi
C- Tli	iii)  Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19841

#Part-A Aptitude & General Biotechnology

Which of the following is true regarding Reverse transcriptase ?