TLS Online TPP Program
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TLS Online TPP Program
#Question id: 18958
#Part-A Aptitude & General Biotechnology
The number of clones to represent this fragment in genomic library with a probability of 95% are
TLS Online TPP Program
#Question id: 18959
#Part-A Aptitude & General Biotechnology
Vectors used for construction of genomic libraries, and their capacity is given match them correctly
| A- λ EMBL series | I - 300kb - 1,400 kb |
| B - Cosmids | II - 45 kb |
| C - BACs and YACs (high capacity vectors) | III - 20kb |
TLS Online TPP Program
#Question id: 18960
#Part-A Aptitude & General Biotechnology
cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library
TLS Online TPP Program
#Question id: 18961
#Part-A Aptitude & General Biotechnology
When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called
TLS Online TPP Program
#Question id: 18962
#Part-A Aptitude & General Biotechnology
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,
2. Their large size makes the handling procedures during cloning tedious and cumbersome
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.
These three problems have been resolved by deleting
TLS Online TPP Program
#Question id: 18964
#Part-A Aptitude & General Biotechnology
Size of agrobacterium can be reduced by binary vector formation; these binary vectors can be formed by phenomenon of