TLS Online TPP Program

#Question id: 4357


Which of the following processes would allow the detection of alternative splicing of transcripts from a given gene?

#Part-A Aptitude & General Biotechnology
  1. Compare the DNA sequence of the given gene to that of a similar gene in a related organism.

  2. Measure the relative rates of transcription of the given gene compared to that of a gene known to be constitutively spliced.

  3. Compare the sequences of different primary transcripts made from the given gene.

  4. Compare the sequences of different mRNAs made from the given gene.

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TLS Online TPP Program

#Question id: 14282

#Part-A Aptitude & General Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

TLS Online TPP Program

#Question id: 14283

#Part-B Specialized Branches in Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14283

#Part-A Aptitude & General Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14318

#Part-B Specialized Branches in Biotechnology

system for manufacture of single-cell protein is Methylophilus methylotrophus. This organism is produced aerobically from methanol with ammonia as nitrogen source. The molecular formula for the biomass is CH 1.6800.36N0.22; these cells contain 6% ash. If the actual yield of biomass from methanol is 42% the thermodynamic maximum, what is the oxygen demand?

TLS Online TPP Program

#Question id: 14331

#Part-B Specialized Branches in Biotechnology

To ensure turbulent conditions and minimum mixing time during agitation with a turbine impeller, the Reynolds number must be at least 104. A stirred laboratory-scale fermenter with a turbine impeller 5 cm in diameter is operated at 800 rpm. If the density of broth being stirred is close to that of water, what is the upper limit for viscosity of the suspension if adequate mixing is to be maintained?

TLS Online TPP Program

#Question id: 14332

#Part-B Specialized Branches in Biotechnology

To ensure turbulent conditions and minimum mixing time during agitation with a turbine impeller, the Reynolds number must be at least 104. A stirred laboratory-scale fermenter with a turbine impeller 5 cm in diameter is operated at 800 rpm. If the density of broth being stirred is close to that of water. The mixing system is scaled up so the tank and impeller are 15 times the diameter of the laboratory equipment. The stirrer in the large vessel is operated so that the stirrer tip speed (tip speed = π N i D i) is the same as in the laboratory apparatus. How does scale-up affect the maximum viscosity.