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TLS Online TPP Program

#Question id: 19865

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

                     COLUMN I

                        COLUMN II

 

A) Enhance efficiency

 

i) Multiplex PCR

 

B) Sequence specific amplification

 

ii) Hot-start PCR

 

C) Mutational analysis

 

iii) Megaprimer PCR

 

 

iv) qPCR with SYBR

 

 

v) AS-PCR

 

 

vi) RLM-RACE

#Part-A Aptitude & General Biotechnology
  1. A-ii, iv ; B-i, vi ; C-iii, v 
  2. A-iii, v ; B-ii, iv ; C-i, vi
  3. A-i, vi ; B-iii, v ; C-ii, iv
  4. A-ii, iv ; B-i, vi ; C-iii, v
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TLS Online TPP Program

#Question id: 13066

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is a codominant marker?
TLS Online TPP Program

#Question id: 22898

#Part-B Specialized Branches in Biotechnology

The PCR technique was developed by 
TLS Online TPP Program

#Question id: 1557

#Part-B Specialized Branches in Biotechnology

which one of these antibody can cross placenta?
TLS Online TPP Program

#Question id: 11190

#Part-B Specialized Branches in Biotechnology

For a neuron with an initial membrane potential at -70 mV, an increase in the movement of potassium ions out of that neuron's cytoplasm would result in the ________.
TLS Online TPP Program

#Question id: 12718

#Part-B Specialized Branches in Biotechnology

primary disruptions could be signaling the plant that a change in environmental conditions has occurred and that it’s time to respond by altering existing pathways or by activating stress-response pathways. At least five different types of stress-sensing mechanisms can be distinguished:

                       

                          COLUMN I

 

 

                              COLUMN II

 

A) Physical sensing

 

 

i) usually results from the detection of by-products that accumulate in cells due to the uncoupling of enzymatic or electron transfer reactions, such as the accumulation of ROS during stress caused by too much light.

 

 

B) Biophysical sensing

 

 

ii) often involves the presence of specialized proteins that have evolved to sense a particular stress; for example, calcium channels that can sense changes in temperature and alter Ca2+ homeostasis.

 

 

C) Metabolic sensing

 

 

iii) refers to modifications of DNA or RNA structure that do not alter genetic sequences, such as the changes in chromatin that occur during temperature stress.

 

 

D) Biochemical sensing

 

 

iv) refers to the mechanical effects of stress on the plant or cell structure, for example, the contraction of the plasma membrane from the cell wall during drought stress.

 

 

E) Epigenetic sensing

 

 

v) might involve changes in protein structure or enzymatic activity, such as the inhibition of different enzymes during heat stress.

 

 

  
Match the correct sequence sensing mechanism during in stress;