Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

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#Question id: 19843

#Part-A Aptitude & General Biotechnology

Real time PCR analyzed various steps such as__

a) Denaturation

b) Annealing

c) Phosphorylation

d) Amplification

e) Methylation

f) Extension

Which of the following steps are correctly analyzed by real time PCR?

TLS Online TPP Program

#Question id: 19843

#Part-A Aptitude & General Biotechnology

Real time PCR analyzed various steps such as__

a) Denaturation

b) Annealing

c) Phosphorylation

d) Amplification

e) Methylation

f) Extension

Which of the following steps are correctly analyzed by real time PCR?

TLS Online TPP Program

#Question id: 19844

#Part-A Aptitude & General Biotechnology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

COLUMN I	COLUMN II
A) SYBR green	i) Annealing
B) Taqman Assay	ii) Denaturation
C) Molecular beacon	iii) pH
	iv) Amplification
	v) Extension

TLS Online TPP Program

#Question id: 19844

#Part-A Aptitude & General Biotechnology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

COLUMN I	COLUMN II
A) SYBR green	i) Annealing
B) Taqman Assay	ii) Denaturation
C) Molecular beacon	iii) pH
	iv) Amplification
	v) Extension

TLS Online TPP Program

#Question id: 19845

#Part-A Aptitude & General Biotechnology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

TLS Online TPP Program

#Question id: 19845

#Part-A Aptitude & General Biotechnology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.