Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

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#Question id: 13042

#Part-A Aptitude & General Biotechnology

For analyzing DNA sequences, two techniques have been developed, one is _________ method frequently termed_________, involving the enzymatic synthesis of DNA and stopping the synthesis at specific nucleotides. While another technique, the __________ method of DNA sequencing developed by ___________ is often used for sequencing small fragments of DNA.

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#Question id: 13043

#Part-A Aptitude & General Biotechnology

In sanger’s sequencing chain termination is facilitated by:

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#Question id: 13044

#Part-A Aptitude & General Biotechnology

How many different types of dNTPs are utilized in a reaction mixture when carrying out sanger’s DNA sequencing ?

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#Question id: 13045

#Part-A Aptitude & General Biotechnology

Maxam Gilbert DNA sequencing causes cleavage of DNA based on:

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#Question id: 13052

#Part-A Aptitude & General Biotechnology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

TLS Online TPP Program

#Question id: 13052

#Part-B Specialized Branches in Biotechnology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?