TLS Online TPP Program
More Questions
TLS Online TPP Program
#Question id: 18847
#Part-A Aptitude & General Biotechnology
You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?
TLS Online TPP Program
#Question id: 18848
#Part-A Aptitude & General Biotechnology
You find that your protein sample loses activity during storage. What can you do about this?
TLS Online TPP Program
#Question id: 18849
#Part-A Aptitude & General Biotechnology
Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?
TLS Online TPP Program
#Question id: 18850
#Part-A Aptitude & General Biotechnology
Which of these chromatography types are suitable as a "capture" step in the purification of non-tagged proteins?
TLS Online TPP Program
#Question id: 18851
#Part-A Aptitude & General Biotechnology
Nickel-NTA (Ni-NTA) chromatography is a popular affinity chromatography method for the purification of histidine-tagged proteins. However, SDS-PAGE of the eluted protein can show bands in addition to your target protein. How might you improve this purification step?
TLS Online TPP Program
#Question id: 18852
#Part-A Aptitude & General Biotechnology
What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?