TLS Online TPP Program

#Question id: 19181


Which of the following statement is incorrect regarding the cloning of PCR products?

#Part-A Aptitude & General Biotechnology
  1. In cloning via restriction enzymes, restriction sites are induced before amplification is carried out
  2. The restriction sites are induced in the primers before annealing
  3. The intermediate molecules are having restriction sites at both ends
  4. The amplified molecules can be cut at both the ends by appropriate enzymes
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TLS Online TPP Program

#Question id: 402

#Part-A Aptitude & General Biotechnology

If the DG'° of the reaction A -> B is –40 kJ/mol, under standard conditions the reaction:

TLS Online TPP Program

#Question id: 404

#Part-B Specialized Branches in Biotechnology

When a mixture of 3-phosphoglycerate and 2-phosphoglycerate is incubated at 25 °C with phosphoglycerate mutase until equilibrium is reached, the final mixture contains six times as much 2-phosphoglycerate as 3-phosphoglycerate.  Which one of the following statements is most nearly correct, when applied to the reaction as written?  (R = 8.315 J/mol·K; T = 298 K)  3-Phosphoglycerate -> 2-phosphoglycerate

TLS Online TPP Program

#Question id: 405

#Part-B Specialized Branches in Biotechnology

When a mixture of glucose 6-phosphate and fructose 6-phosphate is incubated with the enzyme phosphohexose isomerase (which catalyzes the interconversion of these two compounds) until equilibrium is reached, the final mixture contains twice as much glucose 6-phosphate as fructose 6-phosphate.  Which one of the following statements is best applied to this reaction outlined below? (R = 8.315 J/mol·K; T = 298 K) Glucose 6-phosphate -> fructose 6-phosphate

TLS Online TPP Program

#Question id: 406

#Part-B Specialized Branches in Biotechnology

Hydrolysis of 1 M glucose 6-phosphate catalyzed by glucose 6-phosphatase is 99% complete at equilibrium (i.e., only 1% of the substrate remains). Which of the following statements is most nearly correct? (R = 8.315 J/mol·K; T = 298 K)

TLS Online TPP Program

#Question id: 407

#Part-B Specialized Branches in Biotechnology

The reaction A + B -> C has a DG'° of –20 kJ/mol at 25° C.  Starting under standard conditions, one can predict that:

TLS Online TPP Program

#Question id: 409

#Part-B Specialized Branches in Biotechnology

For the following reaction,DG'° = +29.7 kJ/mol. L-Malate + NAD+ -> oxaloacetate + NADH + H+ The reaction as written: