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#Question id: 18985


there are certain limitations of Recombinant Protein Production in E. coli in which transcription terminator of them, to overcome from this

#Part-A Aptitude & General Biotechnology
  1. If such be the case, cDNA of the concerned gene must be used
  2. amino-acid composition
  3. changing terminator sequences
  4. Oligonucleotide mutagenesis could be used to suitably modify the sequence of possible terminator sequences without altering the sequence of encoded amino acids
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