TLS Online TPP Program

#Question id: 18984


there are certain limitations of Recombinant Protein Production in E. coli in which presence of introns are one of them, to overcome from this

#Part-A Aptitude & General Biotechnology
  1. Oligonucleotide mutagenesis could be used to suitably modify the sequence of possible terminator sequences without altering the sequence of encoded amino acids.
  2. changing terminator sequences
  3. If such be the case, cDNA of the concerned gene must be used
  4. amino-acid composition
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TLS Online TPP Program

#Question id: 14281

#Part-B Specialized Branches in Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 


if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

TLS Online TPP Program

#Question id: 14282

#Part-B Specialized Branches in Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

TLS Online TPP Program

#Question id: 14282

#Part-A Aptitude & General Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

TLS Online TPP Program

#Question id: 14283

#Part-B Specialized Branches in Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14283

#Part-A Aptitude & General Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14318

#Part-B Specialized Branches in Biotechnology

system for manufacture of single-cell protein is Methylophilus methylotrophus. This organism is produced aerobically from methanol with ammonia as nitrogen source. The molecular formula for the biomass is CH 1.6800.36N0.22; these cells contain 6% ash. If the actual yield of biomass from methanol is 42% the thermodynamic maximum, what is the oxygen demand?