TLS Online TPP Program

#Question id: 18852


What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?

#Part-A Aptitude & General Biotechnology
  1. Prediction of isoelectric point (pI) from the amino acid sequence
  2. Test protein binding to an IEX matrix at a range of pHs and salt concentrations
  3. Test protein binding to a selection of anion and cation exchange matrices
  4. Pass your sample through a preparative column and elute with a salt gradient