You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?

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#Question id: 11933

#Part-A Aptitude & General Biotechnology

The excitatory or inhibitory action of a neurotransmitter is determined by which of the following?

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#Question id: 13082

#Part-A Aptitude & General Biotechnology

Statement: AFLP shares some features of both RFLP and RAPD analyses.

Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

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#Question id: 18775

#Part-A Aptitude & General Biotechnology

which of the following technique is a highly sensitive and quantitative procedure that utilizes radioactively labelled antigen or antibody

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#Question id: 750

#Part-B Specialized Branches in Biotechnology

In strong acid and at elevated temperatures, for example perchloric acid (HClO4) at more than 100°C, nucleic acids tend to:

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#Question id: 11205

#Part-B Specialized Branches in Biotechnology

What would probably happen if a long neuron had one continuous myelin sheath down the length of the axon with no nodes of Ranvier?