For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed.

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#Question id: 14205

#Part-B Specialized Branches in Biotechnology

steady state condition is maintained in _____

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#Question id: 18748

#Part-A Aptitude & General Biotechnology

Which of the following is not a condition for PCR in single cycle?

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#Question id: 16489

#Part-B Specialized Branches in Biotechnology

The beginning of animal tissue culture can be traced when Arnold showed that 

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#Question id: 13059

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 11631

#Part-B Specialized Branches in Biotechnology

A cross between two green corn plants yields 23 progenies that are green and 13 progenies that are yellow. What is the genotype of the green progeny?